vegfr 3  (R&D Systems)

Journal: Biomolecules

Article Title: Three-Dimensional Histological Characterization of the Placental Vasculature Using Light Sheet Microscopy

doi: 10.3390/biom13061009

Figure Lengend Snippet: Co-staining of whole mount placental tissue for pan-cytokeratin, VEGFR-3, and LYVE-1. The staining for pan-cytokeratin, LYVE-1, and VEGFR-3 revealed a complete and homogenous staining result. Mouse placenta (12.5 dpc) immunostained for cytokeratin ( A ), VEGFR-3 ( B ), LYVE-1 ( C ), and merged overlay ( D ). ( AI – DI ) show unspecific cytokeratin staining in the periphery of the sample and triple-positive cells lining the lumen of spiral arteries (arrowheads) in the mesometrial uterus. ( AII – DII ) show cells expressing cytokeratin and VEGFR-3 but no LYVE-1 expression in the labyrinth. Scale bars: 500 µm.

Article Snippet: The following antibodies and isolectin were used: rabbit monoclonal anti-His antibody, diluted to 1:200 (12698, Cell Signaling Technologies, Danvers, MA, USA); goat polyclonal anti-mouse/rat CD31 antibody, diluted to 1:200 (AF3628, R&D Systems, Minneapolis, MN, USA); rabbit polyclonal anti-mouse VEGFR-3 antibody, diluted to 1:100 (AF743, R&D Systems, Minneapolis, MN, USA); Syrian hamster monoclonal anti-mouse podoplanin (PDPN) antibody, diluted to 1:200 (clone 8.1.1, Invitrogen, Waltham, MA, USA); rabbit polyclonal anti-human PROX-1 antibody, diluted to 1:200 (RT300-052, ReliaTech GmbH, Wolfenbüttel, DE, Germany); mouse monoclonal anti-human pan-Cytokeratin antibody, diluted to 1:100 (AE1/AE3, Santa Cruz Biotechnology, Dallas, TX, USA), mouse IgG1 kappa Isotype Control, diluted to 1:100 (P3.6.2.8.1, Invitrogen, Waltham, MA, USA) mouse monoclonal anti-human α-smooth muscle actin (α-SMA) antibody, CY3 labeled, diluted to 1:100 (1A4, Sigma-Aldrich, St. Louis, MO, USA); Isolectin (GS-IB4, Invitrogen, Waltham, USA); donkey polyclonal anti-goat IgG Alexa Fluor™ 488 antibody, diluted to 1:1000 (A11055, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-mouse IgG Alexa Fluor™ 488 antibody, diluted to 1:1000 (A21202, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-rabbit IgG Alexa Fluor™ 568 antibody, diluted to 1:1000 (A10042, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-goat IgG Alexa Fluor™ 568 antibody, diluted to 1:1000 (A11057, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-rabbit IgG Alexa Fluor™ 647 antibody, diluted to 1:1000 (A11057, Invitrogen, Waltham, MA, USA); and donkey polyclonal anti-Syrian hamster IgG Alexa Fluor™ 647 antibody, diluted to 1:1000 (A78962, Invitrogen, Waltham, MA, USA).

Techniques: Staining, Expressing

Journal: Biomolecules

Article Title: Three-Dimensional Histological Characterization of the Placental Vasculature Using Light Sheet Microscopy

doi: 10.3390/biom13061009

Figure Lengend Snippet: Light sheet images allow a three-dimensional visualization of the stained sample. The whole mount immunofluorescence staining of a mouse placenta (12.5 dpc) for cytokeratin ( A ), LYVE-1 ( B ), VEGFR-3 ( C ), and merged ( D ). Shown are computational volumetric reconstructions, revealing the three-dimensional architecture of the vessels. The zoom-in shows the previously described triple-positive vascular structure ( AI – DI ).

Article Snippet: The following antibodies and isolectin were used: rabbit monoclonal anti-His antibody, diluted to 1:200 (12698, Cell Signaling Technologies, Danvers, MA, USA); goat polyclonal anti-mouse/rat CD31 antibody, diluted to 1:200 (AF3628, R&D Systems, Minneapolis, MN, USA); rabbit polyclonal anti-mouse VEGFR-3 antibody, diluted to 1:100 (AF743, R&D Systems, Minneapolis, MN, USA); Syrian hamster monoclonal anti-mouse podoplanin (PDPN) antibody, diluted to 1:200 (clone 8.1.1, Invitrogen, Waltham, MA, USA); rabbit polyclonal anti-human PROX-1 antibody, diluted to 1:200 (RT300-052, ReliaTech GmbH, Wolfenbüttel, DE, Germany); mouse monoclonal anti-human pan-Cytokeratin antibody, diluted to 1:100 (AE1/AE3, Santa Cruz Biotechnology, Dallas, TX, USA), mouse IgG1 kappa Isotype Control, diluted to 1:100 (P3.6.2.8.1, Invitrogen, Waltham, MA, USA) mouse monoclonal anti-human α-smooth muscle actin (α-SMA) antibody, CY3 labeled, diluted to 1:100 (1A4, Sigma-Aldrich, St. Louis, MO, USA); Isolectin (GS-IB4, Invitrogen, Waltham, USA); donkey polyclonal anti-goat IgG Alexa Fluor™ 488 antibody, diluted to 1:1000 (A11055, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-mouse IgG Alexa Fluor™ 488 antibody, diluted to 1:1000 (A21202, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-rabbit IgG Alexa Fluor™ 568 antibody, diluted to 1:1000 (A10042, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-goat IgG Alexa Fluor™ 568 antibody, diluted to 1:1000 (A11057, Invitrogen, Waltham, MA, USA); donkey polyclonal anti-rabbit IgG Alexa Fluor™ 647 antibody, diluted to 1:1000 (A11057, Invitrogen, Waltham, MA, USA); and donkey polyclonal anti-Syrian hamster IgG Alexa Fluor™ 647 antibody, diluted to 1:1000 (A78962, Invitrogen, Waltham, MA, USA).

Techniques: Staining, Immunofluorescence

Journal: BMC Cancer

Article Title: Lymphatic vessels assessment in feline mammary tumours

doi: 10.1186/1471-2407-7-7

Figure Lengend Snippet: Cat. Mammary gland. VEGFR-3 (red) and laminin (brown) double immunohistochemical stain in an extratumoral area of a stage 0 carcinoma. Lymphatic vessels are laminin negative (asterisk) whereas blood vessels are laminin positive (circle) 20X. Inset: higher magnification of VEGFR-3 positive endothelial cells in a lymphatic vessel. 63X.

Article Snippet: After a step with double staining block solution of the DAKO EnVision™ Doublestain System, the primary polyclonal rabbit anti-mouse FLT-4/VEGFR-3 antibody (Alpha Diagnostic International, San Antonio, Texas, USA) diluted 1:350 in PBS containing 1% of bovine serum albumin was applied for 60 min at room temperature.

Techniques: Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Lymphatic vessels assessment in feline mammary tumours

doi: 10.1186/1471-2407-7-7

Figure Lengend Snippet: Number of lymphatics with and without VEGFR-3 expression in intramammary/intratumoral (A, B) and extramammary/extratumoral (C, D) fields in normal mammary gland (NMG), benign tumours (BT) and malignant tumours (MT).

Article Snippet: After a step with double staining block solution of the DAKO EnVision™ Doublestain System, the primary polyclonal rabbit anti-mouse FLT-4/VEGFR-3 antibody (Alpha Diagnostic International, San Antonio, Texas, USA) diluted 1:350 in PBS containing 1% of bovine serum albumin was applied for 60 min at room temperature.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Lymphatic vessels assessment in feline mammary tumours

doi: 10.1186/1471-2407-7-7

Figure Lengend Snippet: Number of lymphatics with and without VEGFR-3 expression in intratumoral (A, B) and extratumoral (C, D) fields in stage 0, stage I and stage II malignant tumours.

Article Snippet: After a step with double staining block solution of the DAKO EnVision™ Doublestain System, the primary polyclonal rabbit anti-mouse FLT-4/VEGFR-3 antibody (Alpha Diagnostic International, San Antonio, Texas, USA) diluted 1:350 in PBS containing 1% of bovine serum albumin was applied for 60 min at room temperature.

Techniques: Expressing